Regional differences in gene expression for calcium activated neutral proteases (calpains) and their endogenous inhibitor calpastatin in mouse brain and spinal cord

1996 ◽  
Vol 30 (2) ◽  
pp. 177-191 ◽  
Author(s):  
Jinhe Li ◽  
Frida Grynspan ◽  
Stephen Berman ◽  
Ralph Nixon ◽  
Sherry Bursztajn
Keyword(s):  
Gene Expression ◽  
Spinal Cord ◽  
Mouse Brain ◽  
Regional Differences ◽  
Endogenous Inhibitor ◽  
Brain And Spinal Cord ◽  
Neutral Proteases

scholarly journals Characterization of microglial transcriptomes in the brain and spinal cord of mice in early and late experimental autoimmune encephalomyelitis using a RiboTag strategy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shaona Acharjee ◽  
Paul M. K. Gordon ◽  
Benjamin H. Lee ◽  
Justin Read ◽  
Matthew L. Workentine ◽  
...  
Keyword(s):  
Gene Expression ◽  
Spinal Cord ◽  
Experimental Autoimmune Encephalomyelitis ◽  
Expression Patterns ◽  
Immune Functions ◽  
Autoimmune Encephalomyelitis ◽  
Transcriptional Changes ◽  
Brain And Spinal Cord ◽  
The Brain ◽  
Experimental Autoimmune

AbstractMicroglia play an important role in the pathogenesis of multiple sclerosis and the mouse model of MS, experimental autoimmune encephalomyelitis (EAE). To more fully understand the role of microglia in EAE we characterized microglial transcriptomes before the onset of motor symptoms (pre-onset) and during symptomatic EAE. We compared the transcriptome in brain, where behavioral changes are initiated, and spinal cord, where damage is revealed as motor and sensory deficits. We used a RiboTag strategy to characterize ribosome-bound mRNA only in microglia without incurring possible transcriptional changes after cell isolation. Brain and spinal cord samples clustered separately at both stages of EAE, indicating regional heterogeneity. Differences in gene expression were observed in the brain and spinal cord of pre-onset and symptomatic animals with most profound effects in the spinal cord of symptomatic animals. Canonical pathway analysis revealed changes in neuroinflammatory pathways, immune functions and enhanced cell division in both pre-onset and symptomatic brain and spinal cord. We also observed a continuum of many pathways at pre-onset stage that continue into the symptomatic stage of EAE. Our results provide additional evidence of regional and temporal heterogeneity in microglial gene expression patterns that may help in understanding mechanisms underlying various symptomology in MS.

Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord

10.3791/59084 ◽  
2019 ◽  
Author(s):  
David Lutz ◽  
Monika von Düring ◽  
Franco Corvace ◽  
Luzie Augustinowski ◽  
Anne-Kathrin Trampe ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Mouse Brain ◽  
In Utero ◽  
Developing Mouse Brain ◽  
Brain And Spinal Cord

Detection ofmsj-1 gene expression in the frog,Rana esculenta testis, brain, and spinal cord

2004 ◽  
Vol 68 (2) ◽  
pp. 149-158 ◽  
Author(s):  
Rosaria Meccariello ◽  
Gilda Cobellis ◽  
Donatella Scarpa ◽  
Giulia Fienga ◽  
Riccardo Pierantoni ◽  
...  
Keyword(s):  
Gene Expression ◽  
Spinal Cord ◽  
Rana Esculenta ◽  
Brain And Spinal Cord
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